Millipore has launched Immobilon-FL membrane, the first western blotting membrane specifically optimized for fluorescence applications. The background fluorescence of this polyvinylidene fluoride (PVDF) microporous membrane is nearly ten times lower than typical PVDF membranes and 2 to 5 times lower than nitrocellulose membranes. The new membrane offers better sensitivity and improved protein detection with fluorescent reagents.
Immobilon-FL has a 0.45 µm pore size, which is recommended for blotting proteins greater than 20 kDa. It is compatible with standard blocking agents and buffers, as well as all commonly used fluorescent probes. In addition, it can also be used at all excitation and emission wavelengths in both the visible and infrared ranges. It also can be used with chromogenic or chemiluminescent detection methods, according to company sources.
The product is part of Millipore's family of PVDF blotting membranes, including Immobilon-P for general applications and Immobilon-P for blotting small (less than 20 kDa) proteins.
Immobilon is a trademark and Millipore is a registered trademark of Millipore Corporation. Pittcon is a registered trademark of the Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy.