Dolomite Bio has launched a new injection valve and sample loop for single cell RNA sequencing workflows. This option enables straightforward, gentle introduction of evenly distributed mRNA capture beads into the company’s RNA-Seq System for individual encapsulation of cells.
The injection valve and sample loop is a complete module allowing barcoded mRNA capture beads in lysis buffer to be efficiently flowed through the RNA-Seq Chip without clustering or blockages. Bead damage prior to encapsulation is negligible as harsh mechanical stirring is unnecessary, and its four-way valve and simple, compact microfluidic connections enable seamless switching between the injection of the bead suspension and pumping of a driving fluid, with minimal dead volume and bead wastage compared to traditional syringe pump methods. The integrated mirror and chip holder enable easy visualization via the RNA-Seq System’s high speed microscope and camera, and the module also incorporates a Falcon or Eppendorf tube holder for easy collection of the droplet emulsion.
Dolomite Bio creates innovative products for high throughput single cell research. By encapsulating single cells in microfluidic droplets, our products enable rapid analysis of thousands or millions of individual cells and their biological products.