Alnylam Pharmaceuticals, Inc, a leading RNAi therapeutics company, has discovered a key mechanism related to the systemic delivery of RNAi therapeutics using lipid nanoparticles (LNPs). The new pre-clinical research was presented at the 'Advances in Biopharmaceuticals' Keystone Symposium held January 8-13, 2010 in Midway, Utah, and was performed in collaboration with scientists at the Max Planck Institute of Molecular Cell Biology and Genetics. The new data document a key mechanism for endogenous targeting of LNPs to the liver, provide alternative targeting strategies for the hepatic delivery of RNAi therapeutics, and highlight potential targeting approaches for delivery to non-hepatic tissues and cell types.
"A key achievement this past year was our progress in systemic delivery of RNAi therapeutics, the critical scientific determinant for advancement of this promising new class of medicines to patients," said Victor Kotelianski, senior vice president, Distinguished Alnylam Fellow. "As noted recently, one dimension of our progress is evidenced by the discovery of novel LNP compositions that have markedly enhanced potency with efficacy achieved at microgram per kilogram dose levels. Today, we're very pleased to announce our discovery of a key mechanism for systemic delivery by LNPs, a finding that reveals a very promising new frontier for RNAi therapeutics with targeted delivery."
"We're very excited about our new findings as they provide critical mechanistic insights for the continued advancement of LNPs as a platform for systemic delivery of RNAi therapeutics," said Akin Akinc, associate director, Research at Alnylam. "Indeed, we have demonstrated that the endogenous protein apolipoprotein E (ApoE) mediates the liver uptake of certain LNPs in a manner that mimics physiologic mechanisms for lipoprotein metabolism. Further, we've demonstrated the ability to engineer exogenous targeting of LNPs to yet another liver receptor, pointing to the broader opportunity of targeting LNPs to distinct cell types and tissues beyond the liver."
The new in vitro and in vivo research findings establish the role of ApoE as an endogenous targeting ligand for neutrally charged ionizable LNPs (iLNPs), but not certain cationic LNPs (cLNPs), and demonstrate an alternative targeting strategy for the hepatic delivery of RNAi therapeutics using the carbohydrate N-acetylgalactosamine (GalNAc) as an exogenous ligand.
LNP formulations represent one of several approaches Alnylam is pursuing for systemic delivery of RNAi therapeutics. Additional approaches include novel lipidoid formulations, including cLNPs; mimetic lipoprotein particles (MLPs); siRNA conjugation strategies; and single-stranded RNAi; amongst others. Alnylam is currently enrolling patients in a Phase I clinical program with its systemic RNAi therapeutic ALN-VSP for the treatment of liver cancers. In addition, Alnylam intends to initiate a Phase I trial in the first half of 2010 for an additional systemic RNAi therapeutic, ALN-TTR for the treatment of transthyretin (TTR)-mediated amyloidosis. ALN-VSP and ALN-TTR both utilize a first generation LNP formulation known as stable nucleic acid-lipid particles (SNALP), which contains an ionizable lipid, and is developed in collaboration with Tekmira Pharmaceuticals Corp.
RNAi (RNA interference) is a revolution in biology, representing a breakthrough in understanding how genes are turned on and off in cells, and a completely new approach to drug discovery and development.
Alnylam is a biopharmaceutical company developing novel therapeutics based on RNA interference, or RNAi.