SAGE Labs Inc., a leading provider of in vivo products, services and technologies in the field of genome engineering, launchenable researchers to quickly and easily perform genetic engineering in animal models, such as mice and its SAGEspeed CRISPR/Cas9 reagents. These reagents ed rats, as well as cell lines.

Since 2009, SAGE Labs has been using targeted nucleases, including CRISPR/Cas9 and zinc finger nucleases, to perform genetic engineering in mice, rats, and rabbits as part of their SAGEspeed custom model generation service. With the launch of SAGEspeed CRISPR/Cas9 reagents, SAGE is now making available these same custom-designed and validated reagents to the research community.

SAGEspeed CRISPR/Cas9 reagents are available in two different formats. Microinjection-ready reagents come ready-to-inject, prepared in microinjection buffer and at SAGE’s own optimised concentrations for animal model generation. Reagents designed for use in cell lines are also available.

David Smoller, PhD., chief executive officer, of SAGE Labs, explained “CRISPR/Cas9 is an exciting new tool for fast and precise gene editing. While assembly of the CRISPR reagents is relatively simple, design and construction of reagents that perform well can be challenging. Our scientists have amassed a wealth of experience designing and validating nuclease-based gene editing tools for over five years, and we’re tremendously excited to be able to share our expertise with the scientific community by providing the same quality reagents that we use."

SAGEspeed CRISPR/Cas9 reagents are provided at three different levels of validation. SAGEspeed Validated reagents include project-specific design and validation of nuclease activity. SAGE will construct up to five designs and will provide the best performer. Validated+ reagents also include tools to assess potential off target effects.