Waters has introduced new data acquisition mode, SONAR, for its Xevo G2-XS quadrupole time-of-flight (QTof) mass spectrometer (MS) that provides MS/MS data from data-independent acquisitions (DIA).

The technology enables analytical scientists to be more efficient in the laboratory and have more confidence in the results they generate. SONAR data allows for the quantification and identification of lipids, metabolites, and proteins in complex samples from a single injection without additional method development normally associated with MS/MS analysis.

The novel MS acquisition mode was announced at the 15th annual Human Proteome Organization (HUPO) World Congress, where Bob Bateman and John Hoyes, senior MS technology experts at Waters Corporation, were recognized for advancing mass spectrometry instrumentation.

In modern proteomics experiments, DIA-based mass spectrometry has been a popular technique for capturing data-rich sample profiles. As proteomics and lipidomics capabilities have progressed, scientists seek to further focus experiments to quantify specific peptides and proteins, which has required additional method development and repeated assays. The newly introduced SONAR data acquisition mode provides additional clarity and information for increasingly complex samples.

“Today, as proteomics research has matured, scientists are already collecting the majority of the information available from proteins. What they want to be able to do is approach a protein or specific peptides with a hypothesis, and use targeted MS/MS quantification to investigate their ideas without setting up additional methods or experiments,” said David Heywood, senior manager, Omics business development at Waters Corporation.

“Now, with SONAR data acquisition, they can perform a more selective all-in-one analysis. Compatible with high-speed UPLC separations, it’s a more efficient workflow for more accurate qualitative and quantitative analysis within a single injection,” he added.